Dear reader today we talking about a breast cancer research, everybody know that Marfan syndrome is a progressive genetic disorder that affects the body's connective tissue. let me introduction to you the other fact.Peripherally localized aromatase, which converts circulating androgens into estrogens, is important in the pathogenesis of postmenopausal breast cancers. We have previously shown that aromatase mRNA levels are higher in elderly breast cancers (EldCa) than breast cancers of the control group (ContCa) or normal breast tissues. Aromatase expression has been reported to be regulated through the alternative use of multiple exons 1 (exons 1a-1f etc); however, the preferential usage of exons 1 in elderly breast tissue has never been systematically examined. In order to properly treat and protect against EldCa, the regulation mechanism of aromatase expression in elderly breast tissues should be elucidated. The aim of the present study is to elucidate whether there are any specific patterns in use of multiple exons 1 in elderly breast tissue.
Methods
Usage of multiple exons 1 of the aromatase gene and mRNA levels of aromatase were examined by reverse transcription-PCR analysis in breast tissues of 38 elderly patients with breast cancer (age 80-99), and the results were compared with those in 35 patients of the control group (age 37-70). One-factor ANOVA and the Scheffe test were used for the comparison of aromatase mRNA levels. Patterns of preferential utilization of multiple exons 1 of the aromatase gene were compared by Chi-squared test for independence or Fisher's exact test for independence using a contingency table.
Results
Exon 1d was utilized much more frequently in elderly tissue than in the control group irrespective of cancerous or normal tissue (EldCa, 36/38, 95% versus ContCa, 7/35, 20%, P <>1c; EldNorm, all 1c >1d).
Conclusions
Elderly breast tissues exhibited specific patterns in use of multiple exons 1, which at least partly explained the higher aromatase levels in EldCa. The mechanisms of how these specific patterns occur during aging and carcinogenesis should be further examined. hm..i think people you should be have insurance Fraud